Phenotypic Consequences Of Altering Expression Of The Caenorhabditis Elegans Timing Gene Clk 1 PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Phenotypic Consequences Of Altering Expression Of The Caenorhabditis Elegans Timing Gene Clk 1 PDF full book. Access full book title Phenotypic Consequences Of Altering Expression Of The Caenorhabditis Elegans Timing Gene Clk 1.
Author | : Stephanie Felkai |
Publisher | : |
Total Pages | : 68 |
Release | : 1998 |
Genre | : Caenorhabditis elegans |
ISBN | : |
Download Phenotypic Consequences of Altering Expression of the Caenorhabditis Elegans Timing Gene Clk-1 Book in PDF, ePub and Kindle
"clk-1 mutants of the nematode C. elegans have been phenotypically characterized in previous work and found to have decreased rates of development and of periodic adult behaviours, such as defecation, pumping and swimming (Wong et al., 1995). In this study, the defecation periods in older wild type and clk-1 mutant worms were found to be similar despite their differences in young adults. Transgenic strains which express high levels of clk-1 were characterized phenotypically. Over-expression of the clk-1 gene has no observed effect on physiological rates as measured conventionally in young adults but rather has an effect on defecation rates in an age-dependent manner. A proportion of ageing transgenic animals over-expressing clk-1 did not have decreased defecation period, as seen in ageing wild type and clk-1 mutants. This suggests that the action of CLK-1, which functions in controlling physiological rates, is reduced in older wild type worms. As previously characterized, clk-1 mutants have lengthened life spans compared to wild type (Wong et al., 1995). Consistent with this effect, we found transgenic strains with high clk-1 expression to have a shortened average life span. Furthermore, no effect on average life span was found in transgenic control strains in which clk-1 expression was not altered, confirming that the presence of the transgene and the phenotype marking its presence do not influence the observed effects in strains with high clk-1 expression. Together, the findings from both the studies on physiological rates and life span suggest that clk-1 plays a role in determining the rate at which worms age." --
Author | : Yan Meng |
Publisher | : |
Total Pages | : 194 |
Release | : 2000 |
Genre | : Caenorhabditis elegans |
ISBN | : |
Download Genes that Affect Development and Biological Timing in Caenorhabditis Elegans Book in PDF, ePub and Kindle
"In an effort to find new genes involved in development and the biological timing, I carried out a new screen for viable maternal-effect mutations similar in protocol to the previous screen in which 24 such genes have been identified. I screened 10,600 genomes and isolated 6 slow-growing mutations and 5 behavioral and morphological mutations. Another maternal-effect slow-growing mutation is isolated from a screen for both maternal-effect and non maternal-effect slow development mutations. Genetic mapping suggests that none of the seven slow growing mutations corresponds to previously identified genes, so seven new clk genes (clk-4, clk-5, clk-6, clk-7 clk-8, clk-9, clk-10) have been identified. Because most identified clk genes (clk-2, clk-4 to clk-10 and gro-1) are defined by single allele, we believe that these genes have not yet been saturated. Mutants of seven new clk genes have typical Clk phenotypes: a mean lengthening of embryonic development, postembryonic development, defecation cycle and life span. As the screening procedure did not involve any measure of life span, it is suggested that slow life is sufficient for long life. As expected, all seven mutations can be maternally rescued." --
Author | : Donald L. Riddle |
Publisher | : Firefly Books |
Total Pages | : 1252 |
Release | : 1997 |
Genre | : Medical |
ISBN | : 9780879695323 |
Download C. Elegans II Book in PDF, ePub and Kindle
Defines the current status of research in the genetics, anatomy, and development of the nematode C. elegans, providing a detailed molecular explanation of how development is regulated and how the nervous system specifies varied aspects of behavior. Contains sections on the genome, development, neural networks and behavior, and life history and evolution. Appendices offer genetic nomenclature, a list of laboratory strain and allele designations, skeleton genetic maps, a list of characterized genes, a table of neurotransmitter assignments for specific neurons, and information on codon usage. Includes bandw photos. For researchers in worm studies, as well as the wider community of researchers in cell and molecular biology. Annotation copyrighted by Book News, Inc., Portland, OR
Author | : Siegfried Hekimi |
Publisher | : Springer Science & Business Media |
Total Pages | : 254 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 354048003X |
Download The Molecular Genetics of Aging Book in PDF, ePub and Kindle
The molecular genetics of aging or life-span determination is an expanding field. One reason is because many people would consider it desirable if hu man life span could be extended. Indeed, it is difficult not to be fascinated by tales of the life and death of people who have succeeded in living a very long life. Because of this, we have placed at the head of this book the chapter by Perls et al. on Centenerians and the Genetics of Longevity. Perls and his coauthors convincingly argue that, while the average life expectancy might be mostly determined by environmental factors because the average person has an average genotype, extremely long life spans are genetically determined. Of course, studying humans to uncover the genetics of aging is not ideal, not so much because one cannot easily perform experiments as because they live such a long time. This is why most of this book describes the current state of research with model organisms such as yeast, worms, flies, and mice. J aswinski focuses on yeast and how metabolic activity and stress resistance affect the longevity of Saccharomyces cerevisiae. In the process, he discusses the concept of aging as applied to a unicellular organism such as yeast and the importance of metabolism and stress resistance for aging in all organisms.
Author | : Ian A. Hope |
Publisher | : OUP Oxford |
Total Pages | : 306 |
Release | : 1999-12-09 |
Genre | : Science |
ISBN | : 019159198X |
Download C. elegans Book in PDF, ePub and Kindle
Caenorhabditis Elegans has been a popular model organism for biological research for over thirty years and has been used to investigate many aspects of animal development, for example apoptosis, the Hox genes, signal transduction pathways, and the development of the nervous system. It has recently taken on new importance with the publication of the entire genome sequence in 1998. The first chapter gives all the basic information on C. elegans required to use it: it's natural history, anatomy, life cycle, development, and evolution. Information on how to obtain, grow, and maintain C. elegans for use as a model system is given in Chapter 4. Chapters 2 and 3 describe the genome project and show how to use genome sequence information by searching the database for homologues using different search methods and then how to analyse the search data. The next chapter gives the essential practical details of transformation and common uses for the technique. Chapter 6 covers reverse genetics and describes strategies for gene inactivation that are known to work in C elegans: epigenetic inactivation and mutational germ line inactivation. Chapter 7 is designed to help the user analyse phenotype by microscopy and includes Normaski, fluorescence, 4-dimensional, and electron microscopy. Techniques for studying the neurobiology of C. elegans are given in chapter 8. Chapter 9 describes the three commonly used approaches for studying gene expression and Chapter 10 deals with the common methods of molecular biology essential for gene characterization. C. elegans is not the ideal organism for biochemical studies, but chapter 11 describes several procedures for producing biochemically useful quantities of pure tissues. The final chapter is about conventional genetics and details the standard procedures for selfing and crossing; mutagenesis and mutant screening; characterization of mutants; gene mapping; temperature-shift experiments and mosaic analysis. Caenorhabditis Elegans: A Practical Approach will therefore provide all the background information necessary for use of C. elegans as a model system.
Author | : Caleb E. Finch |
Publisher | : |
Total Pages | : 304 |
Release | : 1997-10-29 |
Genre | : Health & Fitness |
ISBN | : |
Download Between Zeus and the Salmon Book in PDF, ePub and Kindle
Demographers and public health specialists have been surprised by the rapid increases in life expectancy, especially at the oldest ages, that have occurred since the early 1960s. Some scientists are calling into question the idea of a fixed upper limit for the human life span. There is new evidence about the genetic bases for both humans and other species. There are also new theories and models of the role of mutations accumulating over the life span and the possible evolutionary advantages of survival after the reproductive years. This volume deals with such diverse topics as the role of the elderly in other species and among human societies past and present, the contribution of evolutionary theory to our understanding of human longevity and intergenerational transfers, mathematical models for survival, and the potential for collecting genetic material in household surveys. It will be particularly valuable for promoting communication between the social and life sciences.
Author | : David R. Riddle |
Publisher | : CRC Press |
Total Pages | : 408 |
Release | : 2007-04-19 |
Genre | : Medical |
ISBN | : 9781420005523 |
Download Brain Aging Book in PDF, ePub and Kindle
Recognition that aging is not the accumulation of disease, but rather comprises fundamental biological processes that are amenable to experimental study, is the basis for the recent growth of experimental biogerontology. As increasingly sophisticated studies provide greater understanding of what occurs in the aging brain and how these changes occur
Author | : Thomas Flatt |
Publisher | : OUP Oxford |
Total Pages | : 506 |
Release | : 2011-05-12 |
Genre | : Science |
ISBN | : 0191621021 |
Download Mechanisms of Life History Evolution Book in PDF, ePub and Kindle
Life history theory seeks to explain the evolution of the major features of life cycles by analyzing the ecological factors that shape age-specific schedules of growth, reproduction, and survival and by investigating the trade-offs that constrain the evolution of these traits. Although life history theory has made enormous progress in explaining the diversity of life history strategies among species, it traditionally ignores the underlying proximate mechanisms. This novel book argues that many fundamental problems in life history evolution, including the nature of trade-offs, can only be fully resolved if we begin to integrate information on developmental, physiological, and genetic mechanisms into the classical life history framework. Each chapter is written by an established or up-and-coming leader in their respective field; they not only represent the state of the art but also offer fresh perspectives for future research. The text is divided into 7 sections that cover basic concepts (Part 1), the mechanisms that affect different parts of the life cycle (growth, development, and maturation; reproduction; and aging and somatic maintenance) (Parts 2-4), life history plasticity (Part 5), life history integration and trade-offs (Part 6), and concludes with a synthesis chapter written by a prominent leader in the field and an editorial postscript (Part 7).
Author | : Jean-Marie Robine |
Publisher | : Springer Science & Business Media |
Total Pages | : 140 |
Release | : 2012-12-06 |
Genre | : Medical |
ISBN | : 3642601006 |
Download The Paradoxes of Longevity Book in PDF, ePub and Kindle
An ever greater number of our contemporaries will reach a very much greater age than their ancestors. Longevity is one of the most fertile fields for paradoxes: it is clear that the same causes do not produce the same effects at the age of ten and at the age of one hundred! On the subject of longevity, the "recipe book" is far from having been written. Nevertheless, the Fondation IPSEN has chosen a few of these paradoxes to discuss and try and explain them.
Author | : Mousumi Debnath |
Publisher | : Springer Science & Business Media |
Total Pages | : 527 |
Release | : 2010-01-29 |
Genre | : Medical |
ISBN | : 9048132614 |
Download Molecular Diagnostics: Promises and Possibilities Book in PDF, ePub and Kindle
A rapid development in diverse areas of molecular biology and genetic engineering resulted in emergence of variety of tools. These tools are not only applicable to basic researches being carried out world over, but also exploited for precise detection of abnormal conditions in plants, animals and human body. Although a basic researcher is well versed with few techniques used by him/her in the laboratory, they may not be well acquainted with methodologies, which can be used to work out some of their own research problems. The picture is more blurred when the molecular diagnostic tools are to be used by physicians, scientists and technicians working in diagnostic laboratories in hospitals, industry and academic institutions. Since many of them are not trained in basics of these methods, they come across several gray areas in understanding of these tools. The accurate application of molecular diagnostic tools demands in depth understanding of the methodology for precise detection of the abnormal condition of living body. To meet the requirements of a good book on molecular diagnostics of students, physicians, scientists working in agricultural, veterinary, medical and pharmaceutical sciences, it needs to expose the reader lucidly to: Give basic science behind commonly used tools in diagnostics Expose the readers to detailed applications of these tools and Make them aware the availability of such diagnostic tools The book will attract additional audience of pathologists, medical microbiologists, pharmaceutical sciences, agricultural scientists and veterinary doctors if the following topics are incorporated at appropriate places in Unit II or separately as a part of Unit-III in the book. Molecular diagnosis of diseases in agricultural crops Molecular diagnosis of veterinary diseases. Molecular epidemiology, which helps to differentiate various epidemic strains and sources of disease outbreaks. Even in different units of the same hospital, the infections could be by different strains of the same species and the information becomes valuable for infection control strategies. Drug resistance is a growing problem for bacterial, fungal and parasitic microbes and the molecular biology tools can help to detect the drug resistance genes without the cultivation and in vitro sensitivity testing. Molecular diagnostics offers faster help in the selection of the proper antibiotic for the treatment of tuberculosis, which is a major problem of the in the developing world. The conventional culture and drug sensitivity testing of tuberculosis bacilli is laborious and time consuming, whereas molecular diagnosis offers rapid drug resistant gene detection even from direct clinical samples. The same approach for HIV, malaria and many more diseases needs to be considered. Molecular diagnostics in the detection of diseases during foetal life is an upcoming area in the foetal medicine in case of genetic abnormalities and infectious like TORCH complex etc. The book will be equally useful to students, scientists and professionals working in the field of molecular diagnostics.