Molecular Beacons Signalling Nucleic Acid Probes Methods And Protocols PDF Download
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| Author | : Andreas Marx |
| Publisher | : Humana Press |
| Total Pages | : 266 |
| Release | : 2010-12-10 |
| Genre | : Medical |
| ISBN | : 9781617377266 |
Download Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols Book in PDF, ePub and Kindle
From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals. The book first offers an introduction to the basic principles of fluorescence and then describes applications of fluorogenic probes in real-time PCR, which currently is the gold standard for quantitative DNA and RNA analysis. Coverage extends the potential of realtime as well as advocates simplifications of the probe technologies. It also presents a new simplified molecular beacon design, EasyBeacons, and demonstrates the utility in DNA methylation profiling.
| Author | : Andreas Marx |
| Publisher | : Humana Press |
| Total Pages | : 266 |
| Release | : 2008-04-03 |
| Genre | : Medical |
| ISBN | : 9781588297006 |
Download Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols Book in PDF, ePub and Kindle
From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals. The book first offers an introduction to the basic principles of fluorescence and then describes applications of fluorogenic probes in real-time PCR, which currently is the gold standard for quantitative DNA and RNA analysis. Coverage extends the potential of realtime as well as advocates simplifications of the probe technologies. It also presents a new simplified molecular beacon design, EasyBeacons, and demonstrates the utility in DNA methylation profiling.
| Author | : Chaoyong James Yang |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 201 |
| Release | : 2013-08-15 |
| Genre | : Science |
| ISBN | : 3642391095 |
Download Molecular Beacons Book in PDF, ePub and Kindle
Molecular Beacons explains working principle of molecular beacons, discusses their design, synthesis, purification and characterization, explores their thermodynamic and kinetic properties, and more importantly, reviews their in vivo and in vitro applications with the emphasis on the design and modification of molecular beacons for in vivo mRNA imaging applications. This book is designed to bring together in a single resource an organized and comprehensive view of molecular beacons and will be a valuable resource for academic, clinical and industrial scientists and graduate students who may consider exploring molecular beacons in their research or practice. Chaoyong James Yang is the Lu Jiaxi Professor of Chemistry at Xiamen University, China. Weihong Tan is a Distinguished Professor of Chemistry and Biomedical Engineering at Hunan University, China and also a University of Florida Distinguished Professor and V. T. and Louis Jackson Professor of Chemistry at the University of Florida, USA.
| Author | : Jamila I. Horabin |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 267 |
| Release | : 2007-09-13 |
| Genre | : Medical |
| ISBN | : 158829692X |
Download Hedgehog Signaling Protocols Book in PDF, ePub and Kindle
In both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body. In addition, many cancers and diseases arise as a result of misregulation of the pathway, which makes it an incredibly important system to understand. This volume examines various methods used in the analysis of the Hedgehog signaling pathway, bringing together different lines of analyses into one accessible and comprehensive text. This volume provides a single source for investigators, presenting several different experimental approaches, which are all varied and broad in nature, as well as procedures for a variety of different model systems. Researchers will find this volume to be an invaluble tool as they work towards deeper understanding of the underlying molecular events that the Hedgehog signaling pathway regulates.
| Author | : Dmitry M. Kolpashchikov |
| Publisher | : Humana Press |
| Total Pages | : 0 |
| Release | : 2013-09-13 |
| Genre | : Medical |
| ISBN | : 9781627035347 |
Download Nucleic Acid Detection Book in PDF, ePub and Kindle
In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.
| Author | : Robert H. Symons |
| Publisher | : CRC Press |
| Total Pages | : 246 |
| Release | : 1989-03-31 |
| Genre | : Medical |
| ISBN | : 9780849349423 |
Download Nucleic Acid Probes Book in PDF, ePub and Kindle
With each chapter written by a distinguished expert in the biochemistry field, this comprehensive volume describes the preparation and use of a variety of radioactive and non-radioactive probes in situations ranging from research laboratories to routine diagnosis laboratories. The enzymatic and chemical techniques for labeling nucleic acid probes with radioisotopes and with non-radioactive ligands and haptens are discussed. Additionally, the associated methods for their detection, the use of these probes in the diagnosis of human and microbial pathogens, of plant viruses and viroids, and of human genetic disorders, as well as in the detection of nucleic acids in tissues and cells by in situ hybridization are presented.
| Author | : Chiquito J. Crasto |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 419 |
| Release | : 2007-11-29 |
| Genre | : Medical |
| ISBN | : 1588297209 |
Download Neuroinformatics Book in PDF, ePub and Kindle
Neuroinformatics presents cutting-edge techniques for the synergistic study of neuroinformatics. The book facilitates the efforts of discovering neuroscience through the sharing of data and the use of computational models. It demonstrates the use of neuroinformatic components as a mechanism for understanding complex disorders. It contains detailed explanations, advantages, and disadvantages of traditional and non-invasive imaging methods.
| Author | : Tilman Heise |
| Publisher | : |
| Total Pages | : 314 |
| Release | : 2020 |
| Genre | : Human genetics |
| ISBN | : 9781071602317 |
Download RNA Chaperones Book in PDF, ePub and Kindle
This book provides a wide spectrum of methods to study RNA chaperones in vitro, at the single molecule level, and protocols useful for cell-based assays. Beginning with a section on a number of bacterial proteins for study, the volume also explores proteins from eukaryotic cells and how to delve into the complex interactions between RNA chaperones and the folding and unfolding of proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Chaperones: Methods and Protocols serves as an ideal guide for scientists and students interested in RNA biology and RNA chaperones. Chapter 3 is available Open Access under a CC-BY 4.0 license via link.springer.com.
| Author | : Ralph Rapley |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 997 |
| Release | : 2000-03-24 |
| Genre | : Science |
| ISBN | : 0896034593 |
Download The Nucleic Acid Protocols Handbook Book in PDF, ePub and Kindle
A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyTM series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field.
| Author | : Kevin D. Bunting |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 306 |
| Release | : 2008-01-31 |
| Genre | : Science |
| ISBN | : 158829868X |
Download Hematopoietic Stem Cell Protocols Book in PDF, ePub and Kindle
This revised edition provides up-to-date protocols developed in the HSC field. A team of leading researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. The book provides detailed flow cytometry protocols for thorough analysis of enriched HSC populations, and offers a variety of transplantation approaches to measure HSC function in vivo. This is a much needed technical resource in the critically important field of stem cell investigation.
