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Modern Methods in Analytical Morphology

Modern Methods in Analytical Morphology
Author: J. Gu
Publisher: Springer Science & Business Media
Total Pages: 441
Release: 2012-12-06
Genre: Science
ISBN: 1461525322

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While advances in modem medicine largely parallel our understanding of morphology, discoveries in morphology are propelled by developments of new tools and means to visualize and measure tissue elements. The invention of dissecting, light, fluorescence and electron microscopes together with advances in labeling and staining techniques are among the stepping stones of morphological progress. Today, we are in an exciting new era when classical morphology is being combined with developments from other disciplines. The combination of morphology and immunology resulted in immunocytochemistry; morphology and molecular biology led to in situ hybridization and in situ PCR. Adding computer science to morphology gave birth to image analysis. Combining laser technology and the microsope evolved into confocal microscope. For more than a decade, modem morphology has continued to develop by merging with other disciplines at a rate that is still gathering momentum, providing exciting and dynamic new frontiers for other biological fields. "Modem Methods in Analytical Morphology," based largely on the "First International Workshop on Modem Methods in Analytical Histochemistry, "is an updated review of the current trends in the field. It covers an extensive array of new technical developments in major disciplines of modem morphology. The authors are not only leaders in their fields but also have extensive "hands on" experience with "bench work. " Their chapters are written in a comprehensive manner including discussion of both theoretical considerations and practical applications to give the readers a broad view of the topics covered.


Analytical Morphology

Analytical Morphology
Author: Jiang Gu
Publisher: Springer Science & Business Media
Total Pages: 298
Release: 2013-12-11
Genre: Science
ISBN: 1461241669

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Morphology Methods

Morphology Methods
Author: Ricardo V. Lloyd
Publisher: Springer Science & Business Media
Total Pages: 435
Release: 2001-06-15
Genre: Medical
ISBN: 1592591906

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The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.


Analytical Morphology

Analytical Morphology
Author: Jiang Gu
Publisher:
Total Pages: 264
Release: 1997-01-01
Genre: Cytochemistry
ISBN: 9783764339579

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This volume is a collection of chapters contributed by leading experts in the emerging field of analytical morphology. Its contents cover a wide range of techniques for morphologic research and diagnosis and it is intended for anyone who wants to keep abreast of the rapid development in this field. Analytical morphology is a contemporary science dealing with the analy- sis of shape, size and color arrangement of cell and tissue components by means of a variety of analytical maneuvers. It differs from conventional mor- phology and histopathology in that it employs methods beyond routine hema- toxylin and eosin or histochemical staining. To a great extent, the advance- ment of analytical morphology is based on new advances in other disciplines, such as immunology, molecular biology, laser technology, microwave tech- nology and computer science. Using these new methods, particular cellular components that would otherwise remain invisible, such as peptides, pro- teins, or nucleotide sequences, are highlighted by visible markers through chemical, physical, immunological or molecular biological reactions.These methods include immunocytochemistry, in situ hybridization, in situ poly- merase chain reaction, antigen retrieval, image analysis, and the like. Analytical morphology is the foundation of contemporary medicine. It provides concrete and visible evidence for many conceptual deductions of other life science disciplines.


Microwave Techniques and Protocols

Microwave Techniques and Protocols
Author: Richard T. Giberson
Publisher: Springer Science & Business Media
Total Pages: 223
Release: 2008-05-09
Genre: Science
ISBN: 1592591280

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Richard Giberson and Richard Demaree, Jr, have collected a wide range of time-saving microwave techniques for processing biological samples for evaluation by many different microscopic methods. Described in step-by-step detail by hands-on researchers, these readily reproducible protocols include both optimized classic methods and such state-of-the art techniques as in vivo labeling, formalin fixation of fresh tissue, vacuum processing, and processing for scanning electron microscopy. Each stand-alone microwave method has been handcrafted by a researcher who regularly uses it to ensure processing success and the brightest quality result.


Morphology, Shape and Phylogeny

Morphology, Shape and Phylogeny
Author: Norman MacLeod
Publisher: CRC Press
Total Pages: 318
Release: 2002-02-07
Genre: Nature
ISBN: 0203165179

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Generally, biologists and mathematicians who study the shape and form of organisms have largely been working in isolation from those who work on evolutionary relationships through the analysis of common characteristics. Increasingly however, dialogue between the two communities is beginning to develop - but other than a handful of journal papers, t


Gold and Silver Staining

Gold and Silver Staining
Author: Gerhard W. Hacker
Publisher: CRC Press
Total Pages: 265
Release: 2002-04-17
Genre: Medical
ISBN: 1420040235

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Immunogold silver staining is one of the most sensitive techniques available for visualizing the location of antibodies and nucleotide probes that have been bounded to specific antigens or to nucleotide sequences. As gold and silver staining continues to advance research in molecular morphology, this book presents the information you need to know a


Theory and Application of Morphological Analysis

Theory and Application of Morphological Analysis
Author: David W. Luerkens
Publisher: CRC Press
Total Pages: 336
Release: 1991-07-24
Genre: Science
ISBN: 9780849367779

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This book is one in a series dedicated to fine particle science and technology. Topics covered in the book include the role of definitions, concepts, hypothesis, and laws; morphological analysis of fine particles and surfaces; analytical three-dimensional representations of particle and surface morphologies; the problem of invariance with respect to rotational transformations, as well as transformations characterized by reflection and inversion; matrix mechanics of particle characterization; and general applications of morphological analysis in other areas of science.


Immunocytochemical Methods and Protocols

Immunocytochemical Methods and Protocols
Author: Lorette C. Javois
Publisher: Springer Science & Business Media
Total Pages: 928
Release: 2008-02-02
Genre: Science
ISBN: 1592592139

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Lorette Javois' timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization.