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Methods of Soil Enzymology

Methods of Soil Enzymology
Author: Richard P. Dick
Publisher: John Wiley & Sons
Total Pages: 416
Release: 2020-01-22
Genre: Technology & Engineering
ISBN: 0891188541

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Methods of Soil Enzymology provides the first comprehensive set of vetted methods for studying enzymes in soils. Readers will especially benefit from the step-by-step explanation of the lab procedures, as well as background information for using these methods effectively and analyzing data. Main topics include activity assays, enzyme extraction, and synthetic enzyme complexes. Each method covered includes background informaton, step-by-step descriptions of the procedure, and special comments regarding nuances, pitfalls, and interpretation of the method. Learn the latest research methods, including enzyme extraction methods and procedures for creating synthetic enzyme complexes, as well as the newest ways to use small-scale and high-throughput methods for enzyme activity assays. Written for the researcher, but welcoming to those new to soil enzymology, the introduction includes conceptual information to orient those who are not familiar with these methods but want to use them. In the tradition of SSSA methods books, Methods of Soil Enzymology features a comprehensive approach with a focus on ease of use.


Soil Enzymology

Soil Enzymology
Author: Girish Shukla
Publisher: Springer Science & Business Media
Total Pages: 392
Release: 2010-10-17
Genre: Science
ISBN: 3642142257

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Soil enzymes are one of the vital key mediators involved in nutrient recycling and the decomposition of organic matter and thereby in maintaining soil quality and fertility. This Soil Biology volume covers the various facets of soil enzymes, such as their functions, biochemical and microbiological properties and the factors affecting their activities. Enzymes in the rhizosphere, in forest soils, and in volcanic ash-derived soils are described. Soil enzymes covered include phosphohydrolases, lignocellulose-degrading enzymes, phenol oxidases, fungal oxidoreductases, keratinases, pectinases, xylanases, lipases and pectinases. Several chapters treat the soil enzymatic activities in the bioremediation of soils contaminated with pesticides and pollutants such as oil, chlorinated compounds, synthetic dyes and aromatic hydrocarbons. The role of soil enzymes as bioindicators is a further important topic addressed.


Methods of Soil Analysis, Part 2

Methods of Soil Analysis, Part 2
Author: Richard W. Weaver
Publisher: ACSESS
Total Pages: 1164
Release: 1994
Genre: Science
ISBN:

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Soil sampling for microbiological analysis; Statistical treatment of microbial data; Soil sterilization; Soil water potencial; Most probable number counts; Light microscopic methods for studying soil microorganisms; Viruses; Recovery and enumeration of viable bacteria; Coliform bacteria; Autotrophic nitrifying bacteria; Free-living dinitrogen-fixing bacteria; Legume nodule symbionts; Anaerobic bacteria and processes; Denitrifiers; Actiomycetes; Frankia and the actinorhizal symbiosis; Filamentous fungi; Vesicular-arbuscular mycorrhizal fungi; Isolation of microorganisms producting antibiotics; Microbiological procedures for biodegradation research; Algae and cyanobacteria; Marking soil bacteria with lacZY; Detection of specific DNA sequences in environmental sample via polymerase chaim reaction; Isolation and purification of bacterial DNA from soil; Microbial biomass; Soil enzymes; Carbon mineralization; Isotopic methods for the study of soil organic matter dynamics ; Practical considerations in the use of nitrogen tracers in agricultural and environmental research; Nitrogen availability; Nitrogen mineralization, immobilization, and nitrification; Dinitrogen fixation; Measuring denitrification in the field; Sulfur oxidation and reduction in soils; Iron and manganese oxidation and reduction.


Soil Enzymology in the Recycling of Organic Wastes and Environmental Restoration

Soil Enzymology in the Recycling of Organic Wastes and Environmental Restoration
Author: Carmen Trasar-Cepeda
Publisher: Springer Science & Business Media
Total Pages: 355
Release: 2011-09-30
Genre: Nature
ISBN: 3642211623

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Soil enzymes play a fundamental role in many soil processes such as the mineralization of organic matter, the synthesis of humic substances, the degradation of xenobiotics or the mechanisms involved in the biocontrol of plant pathogens. Their direct link with soil microorganisms gives them a key role as biomonitors of the evolution of soil quality or in the monitoring of the application of organic amendments to degraded soils. As a consequence of the importance of soil enzymes on soil processes, there is an increasing interest in their study, as well as in the application of molecular techniques as diagnostic tools.


Methods in Soil Biology

Methods in Soil Biology
Author: Franz Schinner
Publisher: Springer Science & Business Media
Total Pages: 438
Release: 2012-12-06
Genre: Science
ISBN: 364260966X

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In terrestrial ecosystems, soil microorganisms and soil animals are essential for litter degradation, soil formation and the availability of nutrients and trace elements. The measurement of biological soil parameters allows a rapid evaluation of the effects of chemical and physical influences due to pollutants or soil management. This book introduces a number of well proved methods for the analysis of carbon, nitrogen, phosphorus and sulfur cycles. It focuses further on the determination of the number and biomass of microorganisms, algae and animals in the soil. Particular emphasis is placed on the comprehensible and complete description of the experimental procedures.


Enzymes in the Environment

Enzymes in the Environment
Author: Richard G. Burns
Publisher: CRC Press
Total Pages: 740
Release: 2002-01-29
Genre: Science
ISBN: 0824744209

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The need to understand the biological processes that are important for essential aquatic and terrestrial ecosystem function has prompted much research into the field of ecological enzymology. This book presents the two broad areas of application in a compilation of reviews by 21 international experts in their respective fields. The first explores enzymatic activities to assess the processes or mechanisms that operate in a given system, such as the rhizosphere, plant leaves and shoots, soil surfaces, and biofilms. The second considers enzymes or microbial cells as sensors to detect microbial activity and stresses due to pollution, management, or climatic change in both aquatic and terrestrial ecosystems.


Methods of Soil Analysis, Part 3

Methods of Soil Analysis, Part 3
Author: D. L. Sparks
Publisher: John Wiley & Sons
Total Pages: 1424
Release: 2020-01-22
Genre: Technology & Engineering
ISBN: 0891188258

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A thorough presentation of analytical methods for characterizing soil chemical properties and processes, Methods, Part 3 includes chapters on Fourier transform infrared, Raman, electron spin resonance, x-ray photoelectron, and x-ray absorption fine structure spectroscopies, and more.


Methods to Determine Enzymatic Activity

Methods to Determine Enzymatic Activity
Author: Alane Beatriz Vermelho
Publisher: Bentham Science Publishers
Total Pages: 334
Release: 2013-11-22
Genre: Science
ISBN: 1608053008

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Methods to Determine Enzymatic Activity is a textbook about industrial enzymes. The book features definitions, classifications and applications of selected enzymes important in industry and in biotechnological processes. Analytical methods for these enzymes are also included in the text. The main objective of this textbook is to provide readers information focused on the current analysis methods of enzymatic activity at qualitative and quantitative levels. Each chapter is about one specific enzyme and contains information about its substrate and some biochemical properties. The methodologies are presented as an experimental protocol allowing interested readers to reproduce the experimental methods detailed within the textbook. These protocols contain the principle of the technique, materials, methods, and all steps necessary for the determination of enzyme activity and interpretation of results. Each methodology is illustrated with photos and schemes for a better and clear understanding. This book, therefore, uniquely brings modern analysis techniques of industrial enzymes in a single easy to understand volume. This textbook is suitable for undergraduate enzymology courses and advanced industrial biotechnology and microbiology courses.


Protocols and Applications in Enzymology

Protocols and Applications in Enzymology
Author: Seema Anil Belorkar
Publisher: Academic Press
Total Pages: 268
Release: 2021-11-19
Genre: Science
ISBN: 0323914373

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Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses. Provides step-by-step instruction on enzyme protocols and applications, with actionable discussions of needed resources, materials, quantification and statistical analysis, optimization and troubleshooting, safety considerations and standards Presents easy to read, reproducible protocols for researchers and students across academia and industry Includes color diagrams that illustrate key concepts


Enzymology of Disturbed Soils

Enzymology of Disturbed Soils
Author: S. Kiss
Publisher: Elsevier
Total Pages: 351
Release: 1998-10-21
Genre: Science
ISBN: 0080531148

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The disturbance of soils, like other phenomena of environmental pollution, encountered in so many areas all over the world, has become a subject of extensive concern and has led to a vast amount of literature in the field of enzymology, too.The book is the first comprehensive and updated review of the enzymological literature on three great categories of disturbed soils, including their remediation. The volume consists of three sections: I. Enzymology of oil-contaminated soils; II. Enzymology of soils affected by industrial emissions; and III, enzymology of technogenic soils. Within each section the reviewed studies are grouped by country.The volume will be of great value to soil scientists, environmental scientists, conservationists, and many other scientists in these and related fields.