Large Scale Mapping Of Genetic Interactions In Saccharomyces Cerevisiae Microform PDF Download
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Author | : Amy Hin Yan Tong |
Publisher | : Library and Archives Canada = Bibliothèque et Archives Canada |
Total Pages | : 474 |
Release | : 2005 |
Genre | : |
ISBN | : 9780494028339 |
Download Large-scale Mapping of Genetic Interactions in Saccharomyces Cerevisiae [microform] Book in PDF, ePub and Kindle
In chapter four, I describe the application of SGA analysis to the large-scale mapping of genetic interactions. A genetic interaction network containing & sim;1000 genes and & sim;4000 interactions was mapped by crossing mutations in 132 different query genes into a set of & sim;4700 viable gene deletion mutants and scoring the double mutant progeny for fitness defects. Network connectivity is predictive of function because interactions often occur among functionally related genes. Genetic interactions are largely orthogonal (non-overlapping) with protein-protein interactions, but genes coding for proteins that occur in the same pathway or complex display similar patterns of genetic interactions. The genetic network shows dense local neighbourhoods, implying the position of a gene on a partially mapped network is predictive of interactions. Because genetic networks are likely conserved, synthetic genetic interactions may underlie the complex genetics associated with inherited phenotypes in other organisms. In chapter three, I describe the development of a new method for automated identification of genetic interactions, termed synthetic genetic array (SGA) analysis. SGA analysis allows systematic construction of double mutants and examination of their fitness on a genome-wide scale. Functional genomics approaches have provided the opportunity for systematic examination of all genes in a genome, generating functional information such as gene expression profiles, protein expression and localization profiles, protein-protein interaction networks, and systematic characterization of mutants. Budding yeast has been the organism of choice for many of these pioneering studies because of its facile genetics. Large-scale studies have made significant contributions to our understanding of complex biological systems, and this trend is continuously fueled by new development of high-throughput technologies. In this thesis, I describe a general strategy to study protein-protein interaction modules (chapter two). A protein-protein interaction network was generated by focusing on yeast SH3 domains and combining data derived from phage-display ligand consensus sequences and large-scale two-hybrid physical interactions. This study produced a network that is depleted of most false positive interactions and enriched for biologically relevant interactions.
Author | : Ian Matthew Donaldson |
Publisher | : National Library of Canada = Bibliothèque nationale du Canada |
Total Pages | : |
Release | : 1999 |
Genre | : |
ISBN | : 9780612457485 |
Download Genetic Interactions Among Components of the Messenger RNA Biogenesis Machinery in the Yeast Saccharomyces Cerevisiae [microform] Book in PDF, ePub and Kindle
Author | : |
Publisher | : DIANE Publishing |
Total Pages | : 215 |
Release | : 1988 |
Genre | : Gene mapping |
ISBN | : 142892258X |
Download Mapping our genes : the genome projects : how big, how fast? Book in PDF, ePub and Kindle
Author | : Klaus Wolf |
Publisher | : Springer Science & Business Media |
Total Pages | : 538 |
Release | : 2003-03-04 |
Genre | : Medical |
ISBN | : 9783540442158 |
Download Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology Book in PDF, ePub and Kindle
Most information on yeasts derives from experiments with the conventional yeasts Saccaromyces cerevisiae and Schizossaccharomyces pombe, the complete nuclear and mitochondrial genome of which has also been sequenced. For all other non-conventional yeasts, investigations are in progress and the rapid development of molecular techniques has allowed an insight also into a variety of non-conventional yeasts. In this bench manual, over 70 practical protocols using 15 different non-conventional yeast species and in addition several protocols of general use are described in detail. All of these experiments on the genetics, biochemistry and biotechnology of yeasts have been contributed by renowned laboratories and have been reproduced many times. The reliable protocols are thus ideally suited also for undergraduate and graduate practical courses.
Author | : Frederick Marcus |
Publisher | : Springer Science & Business Media |
Total Pages | : 296 |
Release | : 2008-07-22 |
Genre | : Science |
ISBN | : 3540783539 |
Download Bioinformatics and Systems Biology Book in PDF, ePub and Kindle
Collaborative research in bioinformatics and systems biology is a key element of modern biology and health research. This book highlights and provides access to many of the methods, environments, results and resources involved, including integral laboratory data generation and experimentation and clinical activities. Collaborative projects embody a research paradigm that connects many of the top scientists, institutions, their resources and research worldwide, resulting in first-class contributions to bioinformatics and systems biology. Central themes include describing processes and results in collaborative research projects using computational biology and providing a guide for researchers to access them. The book is also a practical guide on how science is managed. It shows how collaborative researchers are putting results together in a way accessible to the entire biomedical community.
Author | : National Research Council |
Publisher | : National Academies Press |
Total Pages | : 128 |
Release | : 1988-01-01 |
Genre | : Science |
ISBN | : 0309038405 |
Download Mapping and Sequencing the Human Genome Book in PDF, ePub and Kindle
There is growing enthusiasm in the scientific community about the prospect of mapping and sequencing the human genome, a monumental project that will have far-reaching consequences for medicine, biology, technology, and other fields. But how will such an effort be organized and funded? How will we develop the new technologies that are needed? What new legal, social, and ethical questions will be raised? Mapping and Sequencing the Human Genome is a blueprint for this proposed project. The authors offer a highly readable explanation of the technical aspects of genetic mapping and sequencing, and they recommend specific interim and long-range research goals, organizational strategies, and funding levels. They also outline some of the legal and social questions that might arise and urge their early consideration by policymakers.
Author | : Heinrich Klefenz |
Publisher | : Wiley-VCH |
Total Pages | : 328 |
Release | : 2002-04-22 |
Genre | : Medical |
ISBN | : |
Download Industrial Pharmaceutical Biotechnology Book in PDF, ePub and Kindle
This volume focuses on pharmaceutical biotechnology as a key area of life sciences. The complete range of concepts, processes and technologies of biotechnology is applied in modern industrial pharmaceutical research, development and production. The results of genome sequencing and studies of biological-genetic function are combined with chemical, micro-electronic and microsystem technology to produce medical devices and diagnostic biochips. A multitude of biologically active molecules is expanded by additional novel structures created with newly arranged gene clusters and bio-catalytic chemical processes. New organisational structures in the co-operation of institutes, companies and networks enable faster knowledge and product development and immediate application of the results of research and process development. This book is the ideal source of information for scientists and engineers in research and development, for decision-makers in biotech, pharma and chemical corporations, as well as for research institutes, but also for founders of biotech companies and people working for venture capital corporations.
Author | : |
Publisher | : |
Total Pages | : 528 |
Release | : 1991-07 |
Genre | : Agriculture |
ISBN | : |
Download Bibliography of Agriculture Book in PDF, ePub and Kindle
Author | : K. Esser |
Publisher | : Springer Science & Business Media |
Total Pages | : 396 |
Release | : 1999-11-12 |
Genre | : Science |
ISBN | : 9783540649915 |
Download Progress in Botany Book in PDF, ePub and Kindle
With one volume each year, this series keeps scientists and advanced students informed of the latest developments and results in all areas of the plant sciences. The present volume includes reviews on genetics, cell biology, and vegetation science.
Author | : G. Vidya Sagar |
Publisher | : New Age International |
Total Pages | : 21 |
Release | : 2008 |
Genre | : Medical microbiology |
ISBN | : 8122423523 |
Download MCQs in Microbiology Book in PDF, ePub and Kindle