Introduction To Spatial Mapping Of Biomolecules By Imaging Mass Spectrometry PDF Download
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Author | : Bindesh Shrestha |
Publisher | : Elsevier |
Total Pages | : 272 |
Release | : 2021-04-23 |
Genre | : Science |
ISBN | : 0128189991 |
Download Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry Book in PDF, ePub and Kindle
Imaging mass spectrometry (MS) techniques are often utilized without an understanding of their underlying principles, making it difficult for scientists to determine when and how they can exploit MS to visualize their biomolecules of interest. Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry is an essential reference to help scientists determine the status and strategies of biomolecule analysis, describing its many applications for diverse classes of biomolecules. The book builds a foundation of imaging MS knowledge by introducing ionization sources, sample preparation, visualization guidelines, molecule identification, quantification, data analysis, etc. The second section contains chapters focused on case studies on analyzing a biomolecule class of molecules. Case studies include an introduction/background, and a summary of successful imaging MS studies with illustrative figures and future directions. Provides the introductory foundations of imaging mass spectrometry for those new to the technique Organized by topic to facilitate a quick deep dive, allowing researchers to immediately apply the imaging MS techniques to their work Includes case studies summarizing the imaging MS techniques developed for the class of molecules
Author | : Tiffany Siegel Porta |
Publisher | : Royal Society of Chemistry |
Total Pages | : 541 |
Release | : 2021-12-03 |
Genre | : Science |
ISBN | : 1839162414 |
Download MALDI Mass Spectrometry Imaging Book in PDF, ePub and Kindle
This book gathers knowledge about matrix-assisted laser desorption ionisation (MALDI) mass spectrometry imaging for postgraduate and professional researchers in academia and in industry where it has direct application to clinical research.
Author | : Rudolf Bock |
Publisher | : Walter de Gruyter GmbH & Co KG |
Total Pages | : 642 |
Release | : 2023-07-24 |
Genre | : Science |
ISBN | : 3111182061 |
Download Separation Techniques in Analytical Chemistry Book in PDF, ePub and Kindle
The separation of a mixture into its individual components is one of the most fundamental procedures in analytical and industrial chemistry. This classic book in analytical chemistry provides a comprehensive yet systematic outline of all known separation methods. Through its detailed treatment of the basic principles of separation possibilities, it not only covers what is currently known, but also represents a treasure trove of methods that are still awaiting further development. It is clearly structured and contains interesting examples, further reading and a detailed index. An indispensable book for advanced students of natural sciences (chemistry, biochemistry, food chemistry, pharmacy, clinical chemistry, environmental sciences) and technology (chemical engineering, chemical-physical measurement & biotechnology), as well as teachers of these disciplines.
Author | : Laura M Cole |
Publisher | : Springer Nature |
Total Pages | : 217 |
Release | : 2023-07-06 |
Genre | : Science |
ISBN | : 1071633198 |
Download Imaging Mass Spectrometry Book in PDF, ePub and Kindle
This second edition details new and updated chapters on key methodologies and breakthroughs in the mass spectrometry imaging (MSI) field. Chapters guide readers through nano-Desorption Electrospray Ionisation (nDESI), Matrix Assisted Laser Desorption Ionisation-2 (MALDI-2), Laser Ablation - Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) ,Imaging Mass Cytometry (IMC) with a variety of diverse samples including eye tissue, crop analysis, 3D cell culture models, and counterfeit goods analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Imaging Mass Spectrometry: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Author | : Cheng Guo |
Publisher | : Frontiers Media SA |
Total Pages | : 120 |
Release | : 2022-07-13 |
Genre | : Science |
ISBN | : 2889764362 |
Download Methodological Advances of MALDI Mass Spectrometry-Based Techniques in Organic and Biomedical Analysis Book in PDF, ePub and Kindle
Author | : Yang Xu |
Publisher | : |
Total Pages | : 91 |
Release | : 2012 |
Genre | : Biomolecules |
ISBN | : |
Download Multimodal Spectral Microscopy and Imaging Mass Spectrometry of Biomolecules in Cells and Tissues Book in PDF, ePub and Kindle
Biomolecular imaging enables localization and characterization of biomolecules at the cellular and tissue levels in order to better understand their roles in biological organisms. Although a plethora of imaging techniques is available, there is a need to improve current imaging methodologies and apply them for selective analyses of complex biological specimens. The work described in this thesis is related to development and applications of spectral imaging and imaging mass spectrometry (IMS) methodologies. Spectral imaging uses either labeling dyes or native chromophores to visualize biomolecules in cells and tissues under a specialized light microscope, while IMS provides direct detection and analysis of biomolecules on tissue surfaces with preserved spatial distribution. In this initial study, multimodal spectral imaging of cells, cellular organelles, and tissues was performed on a light microscope upon the addition of a transmission diffraction grating. The instrument concurrently recorded spectral images by fluorescence, darkfield, brightfield, and differential interference contrast (DIC) spectral microscopy. Fluorescence signals originating from fluorescently labeled biomolecules in cells were collected through triple or single filter cubes, separated by the grating, and were imaged using a charge-coupled device (CCD) camera. Cellular components such as nuclei, cytoskeleton, and mitochondria were spatially separated by the fluorescence spectra of the fluorophores present in them, providing detailed multi-colored spectral images of cells. Additionally, the grating-based spectral microscope enabled measurement of scattering and absorption spectra of unlabeled cells and stained tissue sections using darkfield and brightfield spectral microscopy, respectively. In the second project, matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) was utilized to visualize the protein spatial distribution on tissue surfaces. Our study has been focused on imaging of proteins in kidney tissue sections originating from apparently healthy (normal) mice and mice with polycystic kidney disease (PKD). Reconstruction of protein ion maps and identification of proteins by on-tissue enzymatic digestion were performed. MALDI-MS images were correlated with histological features obtained by hematoxylin and eosin staining, providing localization of peptides in different parts of the kidney (renal pelvis, medulla, and cortex). Nano-HPLC was coupled with imaging mass spectrometry to identify proteins in kidney tissue sections. Statistical analyses such as principle component analysis (PCA) was employed to achieve tissue classification and differentiate between healthy and diseased tissues. Experiments are underway to discover and validate proteins that can become potential biomarkers of PKD. In summary, we demonstrated that fluorescence, scattering, and absorption spectral images of cells can be acquired by incorporation of a transmission diffraction grating into a light microscope. The present spectral imaging methodology provides a readily affordable approach for multimodal spectral characterization of biological cells and other specimens. A reproducible MALDI-IMS workflow was established and resulted in protein mass distribution maps of normal and PKD kidney tissues. Statistical comparison of MALDI-MS images provides classification of healthy and diseased tissues. Several proteins were localized in tissues and their identity will be further confirmed by HPLC combined with tandem mass spectrometry (MS/MS).
Author | : Lin He |
Publisher | : Humana |
Total Pages | : 0 |
Release | : 2016-09-22 |
Genre | : Medical |
ISBN | : 9781493954087 |
Download Mass Spectrometry Imaging of Small Molecules Book in PDF, ePub and Kindle
This volume presents new developments in the field of mass spectrometry imaging, covering imaging, software, data analysis, new instrumentations, and new methodological approaches. Chapters provide detailed operational instructions from sample preparation to method selection, from comparative quantification to structural identification and from data collection to visualization of small molecule mapping in complex samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry Imaging of Small Molecules aims to to bring the rapidly maturing methods of metabolic imaging to life science researchers and to minimize technical intimidation in adapting new technological platforms in biological research.
Author | : Joseph Banoub |
Publisher | : Springer |
Total Pages | : 290 |
Release | : 2014-11-03 |
Genre | : Science |
ISBN | : 9401792380 |
Download Detection of Chemical, Biological, Radiological and Nuclear Agents for the Prevention of Terrorism Book in PDF, ePub and Kindle
This NATO-ASI installment is designed to provide an advanced overview for doctoral and post-doctoral candidates of the state-of-the-art technologies for bio-detection. The main objective of the work aims at providing readers with the latest developments necessary to successfully understand the CBRN Agents and their associated biotechnologies. The core methods focused on are mass spectrometry (including chromatographic and electrophoretic separation) and comparisons to spectroscopic, immunological and molecular analysis of chemical, biological and nuclear agents.
Author | : Stanislav S. Rubakhin |
Publisher | : Humana Press |
Total Pages | : 490 |
Release | : 2012-10-13 |
Genre | : Science |
ISBN | : 9781617796968 |
Download Mass Spectrometry Imaging Book in PDF, ePub and Kindle
Mass spectrometry (MS) offers unmatched capabilities for the detection, characterization, and identification of a broad range of analytes. Mass spectrometry imaging (MSI) integrates MS data with information on the spatial distributions of the analytes, further enhancing the applicability of MS. In Mass Spectrometry Imaging: Principles and Protocols, expert practitioners from academia, industry, and the clinic contribute cutting-edge protocols describing the application of MSI to investigations of analyte localization in a variety of specimens, from microorganisms to plant and animal tissues. Divided into three sections, this volume presents the principles of MS, current and future trends of MSI, and qualitative and quantitative protocols to measure and identify endogenous metabolites and xenobiotics. An array of MSI approaches and technologies for characterizing peptide and protein distributions are described in detail. Written in the highly successful Methods in Molecular BiologyTM series format, protocol chapters include introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory procedures. Also included are notes providing tips to avoid experimental pitfalls and helpful suggestions for method troubleshooting. Comprehensive and up-to-date, Mass Spectrometry Imaging: Principles and Protocols is written for scientists, biological and chemical engineers, and clinicians who are interested in applying MSI in their work and those who would benefit from having detailed experimental guidelines available in a single, convenient source.
Author | : Ignacio Rojas |
Publisher | : Springer Nature |
Total Pages | : 485 |
Release | : 2022-06-07 |
Genre | : Science |
ISBN | : 3031078020 |
Download Bioinformatics and Biomedical Engineering Book in PDF, ePub and Kindle
This volume constitutes the proceedings of the 9th International Work-Conference on IWBBIO 2020, held in Maspalomas, Gran Canaria, Spain, in June 2022. The total of 75 papers presented in the proceedings, was carefully reviewed and selected from 212 submissions. The papers cover the latest ideas and realizations in the foundations, theory, models, and applications for interdisciplinary and multidisciplinary research encompassing disciplines of computer science, mathematics, statistics, biology, bioinformatics, and biomedicine.