Fluorescent Methods To Study Biological Membranes PDF Download
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| Author | : Yves Mely |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 484 |
| Release | : 2012-10-10 |
| Genre | : Science |
| ISBN | : 3642331289 |
Download Fluorescent Methods to Study Biological Membranes Book in PDF, ePub and Kindle
Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.
| Author | : Herwig J. Hilderson |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 480 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 1461393590 |
Download Fluorescence Studies on Biological Membranes Book in PDF, ePub and Kindle
As stated by its first editor, Dr. D. B. Roodyn, the primary goal of the series Subcellular Biochemistry is to achieve an integrated view of the cell by bringing together results from a wide range of different techniques and disciplines. This volume deals with the applications of fluorescence spectroscopy to membrane research. It seeks to present complementary biochemical and bio physical data on both the structure and the dynamics of biological membranes. Biophysics and biochemistry are improving more and more in their ability to study biomembranes, overlapping somewhat in this area and explaining the functioning of the whole cell in terms of the properties of its individual com ponents. Therefore, we have brought together an international group of experts in order to report on and review advances in fluorescence studies on biological membranes, thereby highlighting subcellular aspects. The first chapters present a critical evaluation of the current applications of dynamic and steady-state fluorescence techniques. Subsequent chapters dis cuss more specific applications in cells, biological membranes, and their con stituents (lipids, proteins).
| Author | : Radek Šachl |
| Publisher | : Springer Nature |
| Total Pages | : 532 |
| Release | : 2023-04-27 |
| Genre | : Science |
| ISBN | : 3031303628 |
Download Fluorescence Spectroscopy and Microscopy in Biology Book in PDF, ePub and Kindle
This book provides the reader with an updated comprehensive view of the rapidly developing and fascinating field of fluorescence spectroscopy and microscopy. In recent years, fluorescence spectroscopy and microscopy have experienced rapid technological development, which has enabled the detection and monitoring of single molecules with high spatial and temporal resolution. Thanks to these developments, fluorescence has become an even more popular method in physical, biological and related fields. This book guides the reader through both basic and advanced fluorescence spectroscopy and microscopy approaches with a focus on their applications in membrane and protein biophysics. Each of the four parts: A - Fluorescence Spectroscopy, B - Fluorescence Microscopy, C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes and D - Applications of Fluorescence Spectroscopy to protein studies are written by experts within the field. The book is intended for both complete beginners who want to quickly orient themselves in the large number of existing fluorescent methods, as well as for advanced readers who are interested in particular methods and their proper use.
| Author | : Martin Hof |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 315 |
| Release | : 2006-01-27 |
| Genre | : Science |
| ISBN | : 3540270043 |
Download Fluorescence Spectroscopy in Biology Book in PDF, ePub and Kindle
Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.
| Author | : Luca Panconi |
| Publisher | : CRC Press |
| Total Pages | : 137 |
| Release | : 2023-12-21 |
| Genre | : Science |
| ISBN | : 1003823130 |
Download Methods for Imaging Cell Membranes Book in PDF, ePub and Kindle
· Measuring membrane protein distributions using single-molecule localisation microscopy (SMLM) · Measuring membrane protein dynamics and diffusion using fluorescence correlation spectroscopy (FCS) · Mapping membrane lipid backing using environmentally sensitive fluorescence probes · Mapping membrane thickness and rigidity using atomic force microscopy · Mapping membrane proteins and the cytoskeleton using electron microscopy
| Author | : Bruce Alberts |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 2002 |
| Genre | : Cytology |
| ISBN | : 9780815332183 |
Download Molecular Biology of The Cell Book in PDF, ePub and Kindle
| Author | : |
| Publisher | : Academic Press |
| Total Pages | : 428 |
| Release | : 2021-12-01 |
| Genre | : Science |
| ISBN | : 0323911153 |
Download New Methods and Sensors for Membrane and Cell Volume Research Book in PDF, ePub and Kindle
New Methods and Sensors for Membrane and Cell Volume Research, Volume 88 provides an overview of novel experimental approaches to study both the cell membrane and the under-membrane space – the cytosol, which have lately began drawing renewed attention. The book's overall emphasis is on fluorescent and FRET-based sensors, however, other optical (such as variants of transmission microscopy) and non-optical methods (neutron scattering and mass spectrometry) also have dedicated chapters. This volume provides a rare review of experimental approaches to study intracellular phase transitions, as well as anion channels, membrane tension and dynamics, and other topics of intense current interest. Describes novel FRET-based membrane sensors Reviews selected non-optical approaches to membrane structure and dynamics Describes traditional and modern aspects of cell volume research, such as phase transitions and macromolecular crowding
| Author | : Christopher P. Toseland |
| Publisher | : Springer |
| Total Pages | : 306 |
| Release | : 2014-08-05 |
| Genre | : Science |
| ISBN | : 3034808569 |
Download Fluorescent Methods for Molecular Motors Book in PDF, ePub and Kindle
This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments.
| Author | : Gregor Jung |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 287 |
| Release | : 2012-01-05 |
| Genre | : Science |
| ISBN | : 3642233775 |
Download Fluorescent Proteins II Book in PDF, ePub and Kindle
Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008. Fluorescent Proteins II highlights the physicochemical and biophysical aspects of fluorescent protein technology beyond imaging. It is tailored to meet the needs of physicists, chemists and biologists who are interested in the fundamental properties of fluorescent proteins, while also focussing on specific applications. The implementations described are cutting-edge studies and exemplify how the physical and chemical properties of fluorescent proteins can stimulate novel findings in life sciences.
| Author | : Leslie M. Loew |
| Publisher | : CRC Press |
| Total Pages | : 227 |
| Release | : 2018-04-19 |
| Genre | : Science |
| ISBN | : 1351085255 |
Download Spectroscopic Membrane Probes Book in PDF, ePub and Kindle
The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably emerged directly from probe studies over the past 20 years. This work is designed to make these techniques accessible to a board audience of cell biologists. The techniques discussed revolve primarily around the fluorescence of membrane probes, but applications of light absorption and Raman scattering are included. In addition to reviews of the major applications, most chapters include information on the required apparatus, experimental design, data analysis, and potential pit falls.
