Fluorescent Methods For Molecular Motors PDF Download
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| Author | : Christopher P. Toseland |
| Publisher | : Springer |
| Total Pages | : 306 |
| Release | : 2014-08-05 |
| Genre | : Science |
| ISBN | : 3034808569 |
Download Fluorescent Methods for Molecular Motors Book in PDF, ePub and Kindle
This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments.
| Author | : Christopher P. Toseland |
| Publisher | : |
| Total Pages | : 310 |
| Release | : 2014-08-31 |
| Genre | : |
| ISBN | : 9783034808576 |
Download Fluorescent Methods for Molecular Motors Book in PDF, ePub and Kindle
| Author | : Lee Stirling Churchman |
| Publisher | : |
| Total Pages | : 96 |
| Release | : 2007 |
| Genre | : |
| ISBN | : 9780549353676 |
Download Development of Single Molecule Fluorescence Techniques with Application to Molecular Motors Book in PDF, ePub and Kindle
FIONA was employed to follow one motor domain of the dimeric myosin VI molecule. We found that myosin VI moves along actin using a "hand-over-hand" mechanism. SHREC was subsequently developed so that both motor domains of dimeric myosin V could be followed simultaneously. Myosin V was also observed to use a "hand-over-hand" mechanism. To study the kinetics of these dimeric myosins working concentrations approaching the Km of ATP binding to the myosin motor domain would be required (∼1 muM). Linear zero-mode waveguides (lZMW) were devised to reduce the background created by high concentrations of labeled ATP. The lZMW's reduce background by evanescent excitation, nanoconfinement, and limited fluorescence coupling out of the waveguide. Their linearity permits actin filaments to be included in the experimental design. As the collection of single molecule datasets employs specialized equipment and is time intensive, data analysis was optimized by statistical analysis to ensure that the most precise information was extracted.
| Author | : Bruce Alberts |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 2002 |
| Genre | : Cytology |
| ISBN | : 9780815332183 |
Download Molecular Biology of The Cell Book in PDF, ePub and Kindle
| Author | : Mayank Sharma |
| Publisher | : Springer Nature |
| Total Pages | : 328 |
| Release | : 2022-09-15 |
| Genre | : Science |
| ISBN | : 1071626671 |
Download Fluorescent Proteins Book in PDF, ePub and Kindle
This volume brings together cutting-edge laboratory protocols to characterize the novel fluorescent proteins (FPs) and approaches based on fluorescent proteins that aim to answer some of the key cell biological questions. The book covers topics ranging from the database of fluorescent proteins to their characterization and adaptation to a wide range of biological systems. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Fluorescent Proteins: Methods and Protocols serves as an ideal guide for students and academicians enthusiastic about the recent progress in the practical application of fluorescent protein technology.
| Author | : Bernard Valeur |
| Publisher | : John Wiley & Sons |
| Total Pages | : 595 |
| Release | : 2013-03-27 |
| Genre | : Science |
| ISBN | : 3527650024 |
Download Molecular Fluorescence Book in PDF, ePub and Kindle
Molecular Fluorescence This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.
| Author | : Alexander E. Knight |
| Publisher | : Academic Press |
| Total Pages | : 369 |
| Release | : 2009-02-26 |
| Genre | : Science |
| ISBN | : 008092123X |
Download Single Molecule Biology Book in PDF, ePub and Kindle
Single molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. This book provides the first complete and authoritative treatment of this rapidly emerging field, explicitly from a biological perspective.The contents are organized by biological system or molecule. Each chapter discusses insights that have been revealed about their mechanism, structure or function by single molecule techniques. Among the topics covered are enzymes, motor proteins, membrane channels, DNA, ribozymes, cytoskeletal proteins, and other key molecules of current interest. An introduction by the editor provides a concise review of key principles and an historical overview. The last section discusses applications in molecular diagnostics and drug discovery. Organized by biological system or molecule Each chapter discusses insights into mechanism of action, structure, and function Covers enzymes, motor proteins, membrane channels, DNA, ribozymes, etc Includes an introduction to key principles and an historical overview Discusses applications in molecular diagnostics and drug discovery Provides an expert's perspective on future development
| Author | : David M. Jameson |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 459 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 1468456199 |
Download Fluorescent Biomolecules Book in PDF, ePub and Kindle
This volume is based on an international symposium held during September 9-12, 1986 in Bocca di Magra, Italy. The intent of the organizers was to bring together expert practitioners of fluorescence spectroscopy, particularly as applied to biological systems, to assess recent developments in the field and discuss future directions. At the same time the meeting was intended to honor the singular and outstanding scientific career of Gregorio Weber on the occasion of his seventieth birthday. Gregorio Weber is truly the pioneer in the application of fluores cence methods to biochemistry and biophysics. A complete list of his scientific contributions to fluorescence and to protein biochemistry is beyond the scope of this preface. Suffice it to say that since his initial landmark articles on fluorescence, published in the late 1940's and early 1950's, Gregorio Weber has continued to make seminal contribu tions to both the theory and practice of fluorescence and, contrary to many who might be tempted to rest on their laurels, he shows no signs of slackening his pace. In addition to his more obvious tangible contributions to the scientific field, Gregorio Weber has made equally valuable contributions of another type. Specifically, he has had the most profound impact, both profeSSionally and personally, on generations of young scientists.
| Author | : Alberto Diaspro |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 252 |
| Release | : 2010-11-25 |
| Genre | : Science |
| ISBN | : 3642151752 |
Download Optical Fluorescence Microscopy Book in PDF, ePub and Kindle
In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.
| Author | : Barry W. Hicks |
| Publisher | : Humana Press |
| Total Pages | : 384 |
| Release | : 2009-04-01 |
| Genre | : Science |
| ISBN | : 9781934115879 |
Download Viral Applications of Green Fluorescent Protein Book in PDF, ePub and Kindle
Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular BiologyTM series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.
