Dna Cloning A Hands On Approach PDF Download
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Author | : Seok-Yong Choi |
Publisher | : Springer |
Total Pages | : 131 |
Release | : 2019-04-17 |
Genre | : Medical |
ISBN | : 9402416625 |
Download DNA Cloning: A Hands-on Approach Book in PDF, ePub and Kindle
This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.
Author | : Seok-Yong Choi |
Publisher | : |
Total Pages | : 139 |
Release | : 2019 |
Genre | : Clone cells |
ISBN | : 9789402416619 |
Download DNA Cloning Book in PDF, ePub and Kindle
This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.
Author | : David M. Glover |
Publisher | : |
Total Pages | : 208 |
Release | : 1985 |
Genre | : Cloning |
ISBN | : 9780947946180 |
Download DNA Cloning Book in PDF, ePub and Kindle
V.1 - The use of phage lambda replacement vectors in the construction of representative genomic DNA libraries; Constructing and screening cDNA libraries in g10 and gt11; An alternative procedure for the synthesis of double-stranded cDNA for cloning in phage and plasmid vectors; Immunological detection of chimeric B-galactosidases expressed by plasmid vectors; The pEMBL family of single-stranded vectors; Techniques for transformation of E. coli; The use of genetic markers for the selection and allelic exchange of in vitro induced mutation that do not have a phenotype in E. coli; Tle oligonucleotide-directed construction of mutations in recombinant filamentous phage; broad host range cloning vectors for gram negative bacteria; v.2 - Bacillus cloning methods; gene cloning in streptomyces; Cloning in yeast; Genetic engineering of plants; P element mediated germ line transformation of drosophila; High efficiency gene transfer into mammalian cells; The construction and characterization of vaccina virus recombinants expressing foreign genes; Bovine papilloma virus DNA: a eukaryotic cloning vector.
Author | : David M. Glover |
Publisher | : |
Total Pages | : 269 |
Release | : 1995 |
Genre | : |
ISBN | : |
Download DNA Cloning Book in PDF, ePub and Kindle
Author | : David M. Glover |
Publisher | : |
Total Pages | : 269 |
Release | : 1995 |
Genre | : |
ISBN | : |
Download DNA Cloning Book in PDF, ePub and Kindle
Author | : David M. Glover |
Publisher | : |
Total Pages | : |
Release | : 1986 |
Genre | : |
ISBN | : |
Download DNA cloning Book in PDF, ePub and Kindle
Author | : D. M. Glover |
Publisher | : |
Total Pages | : 245 |
Release | : 1985 |
Genre | : |
ISBN | : 9780947946197 |
Download DNA Cloning Book in PDF, ePub and Kindle
Author | : Heather B. Miller |
Publisher | : Academic Press |
Total Pages | : 227 |
Release | : 2011-10-18 |
Genre | : Science |
ISBN | : 0123855454 |
Download Molecular Biology Techniques Book in PDF, ePub and Kindle
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author | : Joseph Sambrook |
Publisher | : |
Total Pages | : 0 |
Release | : 2003 |
Genre | : Eukaryotic cells |
ISBN | : 9780879695774 |
Download Molecular Cloning Book in PDF, ePub and Kindle
Author | : |
Publisher | : Elsevier |
Total Pages | : 405 |
Release | : 2013-09-02 |
Genre | : Science |
ISBN | : 0124199542 |
Download Laboratory Methods in Enzymology: DNA Book in PDF, ePub and Kindle
Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols concentrating on DNA