Characterization Of Glycosylation In Peptides And Proteins By Matrix Assisted Laser Desorption Ionization Mass Spectrometry PDF Download
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Author | : Henrik Rahbek-Nielsen |
Publisher | : |
Total Pages | : |
Release | : 1997 |
Genre | : |
ISBN | : |
Download Characterization of Glycosylation in Peptides and Proteins by Matrix-assisted Laser Desorption Ionization Mass Spectrometry Book in PDF, ePub and Kindle
Author | : John R. Chapman |
Publisher | : Springer Science & Business Media |
Total Pages | : 539 |
Release | : 2008-02-05 |
Genre | : Science |
ISBN | : 1592590454 |
Download Mass Spectrometry of Proteins and Peptides Book in PDF, ePub and Kindle
Little more than three years down the line and I am already writing the Preface to a second volume to follow Protein and Peptide Analysis by Mass . What has happened in between these times to make this second venture worthwhile? New types of mass spectrometric instrumentation have appeared so that new techniques have become possible and existing techniques have become much more feasible. More particularly, however, the newer ionization te- niques, introduced for the analysis of high molecular weight materials, have now been thoroughly used and studied. As a result, there has been an en- mous improvement in the associated sample handling technology so that these methods are now routinely applied to much smaller sample amounts as well as to more intractable samples. Again, this particular community of mass spectrometry users has both increased in number and diversified. And, riding this wave of acceptance, leaders in the field have set their sights on more complex problems: molecular interaction, ion structures, quantitation, and kinetics are just a few of the newer areas reported in Mass Spectrometry of Proteins and Peptides. As with the first volume, one purpose of this collection, Mass Spectr- etry of Proteins and Peptides, is to show the reader what can be done by the application of mass spectrometry, and perhaps even to encourage the reader to venture down new paths.
Author | : Naxing Xu |
Publisher | : |
Total Pages | : 346 |
Release | : 1997 |
Genre | : Biomolecules |
ISBN | : |
Download Structural Characterization of Glycoconjugates and Proteins Book in PDF, ePub and Kindle
Author | : William S. Hancock |
Publisher | : CRC Press |
Total Pages | : 280 |
Release | : 1995-10-23 |
Genre | : Medical |
ISBN | : 9780849378225 |
Download New Methods in Peptide Mapping for the Characterization of Proteins Book in PDF, ePub and Kindle
This text is devoted to the characterization of recombinant DNA-derived proteins by peptide mapping. It describes new technological procedures including capillary electrophoresis, analysis of glycopeptides and the use of electrospray and matrix-assisted laser desorption mass spectrometry. The book presents practical procedures for preparing a protein sample, the enzyme digestion, choice of separation method and procedures for the structural analysis of the separated species. Many figures of peptide maps illustrate typical results. Tables of summary information about digestion, separation conditions, and analyses of important protein samples are also presented.
Author | : John R. Griffiths |
Publisher | : John Wiley & Sons |
Total Pages | : 415 |
Release | : 2016-10-12 |
Genre | : Science |
ISBN | : 1119250889 |
Download Analysis of Protein Post-Translational Modifications by Mass Spectrometry Book in PDF, ePub and Kindle
Covers all major modifications, including phosphorylation, glycosylation, acetylation, ubiquitination, sulfonation and and glycation Discussion of the chemistry behind each modification, along with key methods and references Contributions from some of the leading researchers in the field A valuable reference source for all laboratories undertaking proteomics, mass spectrometry and post-translational modification research
Author | : A.L. Burlingame |
Publisher | : Gulf Professional Publishing |
Total Pages | : 482 |
Release | : 2005-12-13 |
Genre | : Science |
ISBN | : 9780121828103 |
Download Mass Spectrometry: Modified Proteins and Glycoconjugates Book in PDF, ePub and Kindle
This volume provides comprehensive treatment of tools and proper usage for the identification of proteins, affinity chromatography and studies the complexity of protein machines and assemblages, assignment of the most common protein posttranslational modifications (phosphorylation and glycosylation) and glycolipidomics. *Part 2 of 2 volumes about Mass Spectrometry *Discusses peptide and protein cleanup and preparation requirements for mass spectrometry *Explains protein enzymic and chemical digestion strategies *Includes case studies of protein assemblages and machines
Author | : Guodong Chen |
Publisher | : Springer Science & Business Media |
Total Pages | : 408 |
Release | : 2014-07-08 |
Genre | : Science |
ISBN | : 1441978623 |
Download Characterization of Protein Therapeutics using Mass Spectrometry Book in PDF, ePub and Kindle
This book highlights current approaches and future trends in the use of mass spectrometry to characterize protein therapies. As one of the most frequently utilized analytical techniques in pharmaceutical research and development, mass spectrometry has been widely used in the characterization of protein therapeutics due to its analytical sensitivity, selectivity, and specificity. This book begins with an overview of mass spectrometry techniques as related to the analysis of protein therapeutics, structural identification strategies, quantitative approaches, followed by studies involving characterization of process related protein drug impurities/degradants, metabolites, higher order structures of protein therapeutics. Both general practitioners in pharmaceutical research and specialists in analytical sciences will benefit from this book that details step-by-step approaches and new strategies to solve challenging problems related to protein therapeutics research and development.
Author | : Ganglong Yang |
Publisher | : Frontiers Media SA |
Total Pages | : 231 |
Release | : 2022-03-14 |
Genre | : Science |
ISBN | : 2889746704 |
Download Protein Glycosylation – Advances in Identification, Characterization and Biological Function Elucidation using Mass Spectrometry Book in PDF, ePub and Kindle
Author | : J. R. Chapman |
Publisher | : Springer Science & Business Media |
Total Pages | : 368 |
Release | : 1996-08-19 |
Genre | : Medical |
ISBN | : |
Download Protein and Peptide Analysis by Mass Spectrometry Book in PDF, ePub and Kindle
Leading practitioners authoritatively describe the newest and most effective spectrometric techniques for the analysis of proteins and peptides. The areas covered range from the elucidation of primary and secondary protein structure and the rapid identification of proteins using database techniques to methods for sequencing, as well as methods for the quantitative determination of peptides. Other chapters provide detailed information on the analysis of glycoproteins and glycopeptides and on the use of mass spectrometry to probe the interactions of proteins, both covalent and noncovalent.
Author | : Chun-Yu Chan |
Publisher | : |
Total Pages | : 0 |
Release | : 2017-01-26 |
Genre | : |
ISBN | : 9781361207338 |
Download Mass Spectrometric Analysis of Selected Glycoproteins Book in PDF, ePub and Kindle
This dissertation, "Mass Spectrometric Analysis of Selected Glycoproteins" by Chun-yu, Chan, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled MASS SPECTROMETRIC ANALYSIS OF SELECTED GLYCOPROTEINS Submitted by Chan Chun Yu for the degree of Master of Philosophy at The University of Hong Kong in February 2005 Fetuin from fetal calf serum (Sigma, F6131), a well-characterized glycoprotein that has a molecular weight of 48.4 kDa and 3 N-glycosylation sites, was used as a prototypical protein to develop mass spectrometric approaches toward the identification and sequence elucidation of the N-glycosylation sites of proteins. Fetuin was digested using trypsin (porcine) and deglycosylated using peptide N-glycosidase F (PNGase-F) prior to matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometric analysis. For peptide mass fingerprinting, the experimental peak list obtained from the MALDI-TOF mass spectrum of the sample was compared with the theoretical peak list obtained from the National Center for Biotechnology Information sequence database. The identification of each N-glycosylation site was based on the 0.98-Da mass increase observed upon hydrolysis by the amidase PNGase-F of the glycosylamine linkage of each N-glycosylated asparagine, which formed an aspartic acid residue. The identities of the glycopeptides were verified through isotopic pattern analysis using the proposed amino acid composition and the ISOPRO shareware program. All the 3 potential N-glycosylation sites of fetuin were identified successfully in accordance with published results. The peptide sequence coverage was 75%. The developed strategy was then applied to map the N-glycosylation sites of the severe acute respiratory syndrome spike S glycoprotein, a novel glycoprotein that requires a more complex analysis for its total 23 potential N-glycosylation sites. The proposed peptide sequence and its potential N-glycosylation sites were elucidated using tandem mass spectrometry under low-energy collision-induced dissociation. The partial peptide sequence was determined by computing the differences in the mass-to-charge ratios between consecutive b- or y- fragment ions. The sequence tags generated this way were used to confirm the exact sites of N-glycosylation. The effects that the sequential specific enzymatic digestion process, the MALDI matrix, the sample clean-up process, and the enrichment methods have on protein sequence coverage were also examined. For the spike S glycoprotein, a sequence coverage of 52% was obtained. Within the covered peptide sequences, 17 of the 23 potential sites were detected (except for N7, 8, 13, 15, 16, and 20); 6 of them were novel N-glycosylation sites. DOI: 10.5353/th_b3147942 Subjects: Glycoproteins - Analysis Spectrum analysis