Basic Techniques In Molecular Biology PDF Download
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Author | : Stefan Surzycki |
Publisher | : Springer Science & Business Media |
Total Pages | : 442 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 3642569684 |
Download Basic Techniques in Molecular Biology Book in PDF, ePub and Kindle
This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Author | : Heather B. Miller |
Publisher | : Academic Press |
Total Pages | : 227 |
Release | : 2011-10-18 |
Genre | : Science |
ISBN | : 0123855454 |
Download Molecular Biology Techniques Book in PDF, ePub and Kindle
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author | : Leonard G. Davis |
Publisher | : |
Total Pages | : 404 |
Release | : 1992 |
Genre | : Science |
ISBN | : |
Download Basic Methods in Molecular Biology Book in PDF, ePub and Kindle
The new edition of this popular book emphasizes the decisions that need to be made to select one procedure over another.
Author | : Walt Ream |
Publisher | : Academic Press |
Total Pages | : 245 |
Release | : 1998-11-17 |
Genre | : Science |
ISBN | : 0080536824 |
Download Molecular Biology Techniques Book in PDF, ePub and Kindle
This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Provides well-tested experimental protocols for each technique Lists the reagents and preparation of each experiment separately Contains a complete schedule of experiments and the preparation required Includes study questions at the end of each chapter
Author | : J.M. Walker |
Publisher | : Springer Science & Business Media |
Total Pages | : 304 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 9401165637 |
Download Techniques in Molecular Biology Book in PDF, ePub and Kindle
The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.
Author | : Khalid Z. Masoodi |
Publisher | : Academic Press |
Total Pages | : 220 |
Release | : 2020-10-28 |
Genre | : Science |
ISBN | : 012824450X |
Download Advanced Methods in Molecular Biology and Biotechnology Book in PDF, ePub and Kindle
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Author | : Leonard G. Davis |
Publisher | : |
Total Pages | : 406 |
Release | : 1986 |
Genre | : Science |
ISBN | : |
Download Basic Methods in Molecular Biology Book in PDF, ePub and Kindle
Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology-the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three introductory ones that discuss the basics of molecular biology; the tools of the molecular biologist; an ...
Author | : Keith Wilson |
Publisher | : Cambridge University Press |
Total Pages | : 769 |
Release | : 2010-03-04 |
Genre | : Medical |
ISBN | : 0521516358 |
Download Principles and Techniques of Biochemistry and Molecular Biology Book in PDF, ePub and Kindle
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Author | : Surzycki |
Publisher | : |
Total Pages | : 434 |
Release | : 2007-10-01 |
Genre | : |
ISBN | : 9788181287960 |
Download Basic Techniques In Molecular Biology Book in PDF, ePub and Kindle
Author | : Leonard Davis |
Publisher | : Elsevier |
Total Pages | : 401 |
Release | : 2012-12-02 |
Genre | : Science |
ISBN | : 044460149X |
Download Basic Methods in Molecular Biology Book in PDF, ePub and Kindle
Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology—the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three introductory ones that discuss the basics of molecular biology; the tools of the molecular biologist; and general preparations, procedures, and considerations for use of the book. The following chapters then discuss cloning vectors and bacterial cells; preparation of DNA from eukaryotic cells; probing nucleic acids; plasmid DNA preparation; DNA restriction fragment preparation; purification of DNA; and preparation and analysis of RNA from eukaryotic cells. Other chapters cover preparation of DNA from bacteriophage clones; cloning DNA from the eukaryotic genome; subcloning into plasmids; M13 cloning and sequencing; further characterization of cloned DNA; transfection of mammalian cells in culture; protein methods; general methods; and specialized methods. This book will be of interest to practitioners in the fields of biology and molecular genetics.